Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei
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چکیده
منابع مشابه
Enhanced Expression of Endochitinase in Trichoderma harzianum with the cbh1 Promoter of Trichoderma reesei.
Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochi...
متن کاملRole of the Trichoderma harzianum endochitinase gene, ech42, in mycoparasitism.
The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown under inducing conditions increased ...
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The mesophilic filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) has become a major cell factory in the enzyme industry, and the benchmark organism for production of cellulases, especially for biomass conversion. The strain was originally isolated during the second World War from US Army tent canvas in the Solomon Islands and initially identified as Trichoderma viride. The is...
متن کاملOptimization of Extracellular Cellulase Production by Trichoderma harzianum
ABSTRACT Background and Objectives: Cellulose is a major component of plant biomass, which can be converted into biofuels and valuable chemicals. The key step in utilization of this organic material is its hydrolysis into soluble sugars. This study evaluated cellulase production by Trichoderma harzianum under different pH values, temperatures and incubation...
متن کاملCellulase Production by Trichoderma reesei using Sugar Beet Pulp
Cellulase production by the fungus Trichoderma reesei was studied using sugar beet pulp (SBP) as a substrate. The subculture medium was a salt solution consisting of KH2PO4, CaCl2, etc. Fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°C for 1-2 generations (two days for each generation) and were then used as an inoculum. Exponential cells were inoculated into a medium contain...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1996
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.62.6.2145-2151.1996